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This study is a methodological examination of the steps and processes involved in setting up the Gojjam Lymphoedema Best Practice Trial (GoLBeT; ISRCTN67805210), a highly pragmatic clinical trial conducted in northern Ethiopia. Challenges to the trial and strategies used to deal with them were explored, together with reasons for delays.
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competing flora.6 BCYE w/ GPCV is also recommended by Centers for Disease Control and Prevention (CDC) for testing water samples.7 PRINCIPLE BCYE w/ GPCV contains charcoal and yeast extract to enhance the growth of Legionella. Charcoal also serves to absorb toxic metabolic products and modify the surface tension of the medium.
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In GoLBeT, there was a much greater overlap of steps than in the Global Health Research Process Map, in which steps proceed in a sequential, stepwise manner. In GoLBeT, steps often ran alongside each other, and were frequently developed together, along with key documents. For example, the GoLBeT funding proposal was developed alongside the budget.
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BCYE Agar with GPVC (Cat. no. W169) contains glycine, vancomycin, polymyxin and cycloheximide for the greater inhibition of secondary microflora and further selective isolation of Legionella spp. BCYE Agar with CCVC (Cat. no. G170) contains cephalothin, colistin, vancomycin, and cycloheximide. This is based on the formulation of Bopp et al.(14.
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BCYE agar, or Buffered Charcoal Yeast Extract agar, is a specialized bacterial culture medium designed for isolating and cultivating Legionella species, bacteria responsible for causing Legionnaires' disease. It contains charcoal to neutralize inhibitory substances, yeast extract for nutrients, and buffered salts to maintain a stable pH.
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BD BBL™ BCYE (Buffered Charcoal Yeast Extract) Agar is used for the isolation and cultivation of Legionella species. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below. a. Add 0.1 mL of a culture containing 30-300 CFU/0.1 mL to each plate and spread-inoculate using a sterile glass spreader.
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Author summary Podoconiosis is a disease of the lymphatic vessels of the lower extremities, caused by chronic exposure to irritant soils. It results in leg swelling, commonly complicated by acute dermatolymphangioadenitis (ADLA, or 'acute attacks'), characterised by severe pain, fever and disability. The Gojjam Lymphoedema Best Practice Trial (GoLBeT) trial is a successfully completed.
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Goblet cells are unicellular intraepithelial mucin-secreting glands scattered within simple epithelia, such as cuboidal, columnar, and pseudostratified epithelia. Their name corresponds to their shape, as they resemble a goblet, with their narrow bases and wide apex. Their role is to protect the surface of epithelium, lubricate it, and catch.
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Example of a BCYE agar plate with visible colonies of Legionella after... Download Scientific
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